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犬副流感病毒D121004株的分离鉴定及其F基因遗传进化分析

点击数:4276  时间:2019-03-04 14:58:58  来源: 中国动物检疫      作者: 李 超等

为了给犬副流感病毒(CPIV)疫苗研制提供支持,无菌采集有呼吸道症状病死犬的脑和肺脏样品,处理后接种Marc-145细胞,通过RT-PCR、红细胞吸附试验,对盲传3代的细胞培养液进行鉴定,成功分离到1CPIV。该病毒能使Marc-145细胞出现细胞病变,且具有吸附鸡红细胞的能力,将其命名为CPIV-D121004株。对该毒株的F糖蛋白全基因进行克隆及核苷酸同源性分析并构建遗传进化树,发现分离株的F基因较为保守,未出现大的遗传变异。今后需要对分离株的免疫原性进行研究,以探究其作为候选疫苗株的潜力。


Isolation and Identificationof Canine Parainfluenza Virus D121004 Strain

and Sequence Analysis on ItsF Gene

In order to research and develop a vaccineagainst canine parainfluenza virusCPIV),the brains and lungs of dead dogs suspicious of respiratory symptomswere collected asepticallyand then Marc-145 cells were inoculated after treatment to isolateCPIV. RT-PCR and erythrocyte adsorption assay were applied to identify the cellculture medium with three blind passages. The results showed that one strain ofcanine parainfluenza virusCPIV-D121004was successfully isolatedwhich could cause cytopathic changes in Marc-145 cellsand was capable of adsorbing chicken erythrocytes. Cloning and nucleotide homologyanalysis on the whole F glycoprotein gene were carried outand the genetic evolution tree was built.The results showed that the F gene of the isolated strain was relativelyconservativeindicating no major genetic variation. Infuturethe immunogenicity of the isolate should befurther studiedso as to explore the potential to be thevaccine strain.

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