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I群禽腺病毒的分离鉴定与同源性分析

点击数:3720  时间:2019-03-04 14:56:11  来源: 中国动物检疫      作者: 矫海婷等

为了解鸡群中I群禽腺病毒(FAdV-I)的流行现状,从山东、黑龙江、吉林等地部分大规模养鸡场采集病料,进行病毒分离、PCR鉴定及同源性分析。结果显示:从76份病料中分离到10FAdV-I,分离率为13%10FAdV-I中,1株为血清4型,6株为血清8b型,1株为血清9型,2株为血清11型。结果表明,山东、黑龙江、吉林等地均存在一定的FAdV-I流行,主要流行血清型已由4型转变为8b型,因此需要评估当前的4型灭活苗能否有效防控这些地区的FAdV-I流行。


Isolation and Identificationand Homology Analysis on Group I Fowl Adenovirus

In order to recognize the prevalence ofgroup I fowl adenovirusFAdV-Iin poultrysamples were collected from several large-scalechicken farms in ShandongHeilongjiang and Jilin provinces for FAdV isolationPCR assay and homology analysis. Resultsshowed that 10 FAdV-I strains were isolated from 76 samples with the positiverate reaching 13%. Among the 10 FAdV-I strains1 of them belonged to serotype 46 of them belonged to serotype 8b1 of them belonged to serotype 9and 2 of them belonged to serotype 11. Theresults indicated that certain FAdV-I infection existed in ShandongHeilongjiang and Jilin provincesand the main serotypes had been transferredfrom serotype 4 to serotype 8bthus the current serotype 4 inactivated vaccines need to be evaluatedto determine if they could control the spread of FAdV-I effectively.

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